Abstract
A major regulatory function has been evidenced here for HSF1, the key transcription factor of the heat-shock response, in a large-scale remodeling of the cell epigenome. Indeed, upon heat shock, HSF1, in addition to its well-known transactivating activities, mediates a genome-wide and massive histone deacetylation. Investigating the underlying mechanisms, we show that HSF1 specifically associates with and uses HDAC1 and HDAC2 to trigger this heat-shock-dependent histone deacetylation. This work therefore identifies HSF1 as a master regulator of global chromatin acetylation and reveals a cross-talk between HSF1 and histone deacetylases in the general control of genome organization in response to heat shock.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Animals
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Cell Line
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Chromatin / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation*
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Genome*
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Heat Shock Transcription Factors
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Heat-Shock Response / genetics*
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Histone Deacetylase 1 / genetics
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Histone Deacetylase 1 / metabolism
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Histone Deacetylase 2 / genetics
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Histone Deacetylase 2 / metabolism
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Histones / genetics
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Histones / metabolism
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Humans
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Mice
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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Chromatin
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DNA-Binding Proteins
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HSF1 protein, human
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Heat Shock Transcription Factors
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Histones
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Hsf1 protein, mouse
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RNA, Small Interfering
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Transcription Factors
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Histone Deacetylase 1
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Histone Deacetylase 2