Accumulation of DC in lamina propria induced by FMS-like tyrosine kinase 3 ligand aggravates the intestinal inflammatory response during endotoxemia

Inflammation. 2010 Feb;33(1):34-45. doi: 10.1007/s10753-009-9156-9.

Abstract

It is known that the loss of DC plays an important role for immune suppression during endotoxemia or sepsis. To verify our hypothesis that pre-enrichment of the lamina propria (LP) DC pool may improve protective immunity to bacterial translocation and outcome in endotoxemic mice, we pre-treated mice with Flt3L or normal saline, and then challenged them with or without LPS. Twelve hours later the population size and maturity of DC in the LP and circulation were analyzed by flow cytometry. Bacterial translocation to distant organs, inflammatory responses in the intestine and the survival rate of mice were evaluated. We observed that pretreatment of Flt3L significantly expanded DC in the LP and blood, but did not alter their maturation. However, exacerbation of DC growth induced by Flt3L-pretreatment aggravated intestinal inflammation and increased the mortality of endotoxemic mice rather than enhancing their resistance to bacterial translocation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Translocation / drug effects
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Chemokines / metabolism
  • Chemotaxis / drug effects
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology
  • Dendritic Cells / microbiology
  • Disease Models, Animal
  • Endotoxemia / chemically induced
  • Endotoxemia / immunology*
  • Endotoxemia / pathology
  • Escherichia coli / metabolism
  • Escherichia coli / pathogenicity
  • Female
  • Flow Cytometry
  • Green Fluorescent Proteins / biosynthesis
  • Green Fluorescent Proteins / genetics
  • Ileitis / immunology*
  • Ileitis / microbiology
  • Ileitis / pathology
  • Ileum / drug effects*
  • Ileum / immunology
  • Ileum / microbiology
  • Ileum / pathology
  • Immunity, Mucosal / drug effects
  • Injections, Intraperitoneal
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / immunology
  • Lipopolysaccharides
  • Membrane Proteins / administration & dosage*
  • Membrane Proteins / adverse effects
  • Membrane Proteins / immunology
  • Mice
  • Mice, Inbred BALB C
  • Receptors, Chemokine / metabolism
  • Recombinant Proteins / administration & dosage
  • Time Factors
  • Transfection

Substances

  • Chemokines
  • Lipopolysaccharides
  • Membrane Proteins
  • Receptors, Chemokine
  • Recombinant Proteins
  • flt3 ligand protein
  • Green Fluorescent Proteins