Synthesis and characterization of hydroxyl-functionalized caprolactone copolymers and their effect on adhesion, proliferation, and differentiation of human mesenchymal stem cells

Biomacromolecules. 2009 Nov 9;10(11):3048-54. doi: 10.1021/bm900693p.

Abstract

The aim of this study was to develop new hydrophilic polyesters for tissue engineering applications. In our approach, poly(benzyloxymethyl glycolide-co-epsilon-caprolactone)s (pBHMG-CLs) were synthesized through melt copolymerization of epsilon-caprolactone (CL) and benzyl-protected hydroxymethyl glycolide (BHMG). Deprotection of the polymers yielded copolymers with pendant hydroxyl groups, poly(hydroxymethylglycolide-co-epsilon-caprolactone) (pHMG-CL). The synthesized polymers were characterized by GPC, NMR, and DSC techniques. The resulting copolymers consisting of up to 10% of HMG monomer were semicrystalline with a melting temperature above body temperature. Water contact angle measurements of polymeric films showed that increasing HMG content resulted in higher surface hydrophilicity, as evidenced from a decrease in receding contact angle from 68 degrees for PCL to 40 degrees for 10% HMG-CL. Human mesenchymal stem cells showed good adherence onto pHMG-CL films as compared to the more hydrophobic PCL surfaces. The cells survived and were able to differentiate toward osteogenic lineage on pHMG-CL surfaces. This study shows that the aforementioned hydrophilic polymers are attractive candidates for the design of scaffolds for tissue engineering applications.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caproates / chemical synthesis*
  • Caproates / chemistry
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Cell Differentiation* / drug effects
  • Cell Differentiation* / physiology
  • Cell Proliferation* / drug effects
  • Cells, Cultured
  • Humans
  • Hydroxyl Radical / chemical synthesis*
  • Hydroxyl Radical / chemistry
  • Lactones / chemical synthesis*
  • Lactones / chemistry
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / physiology
  • Polymers / chemical synthesis*
  • Polymers / chemistry

Substances

  • Caproates
  • Lactones
  • Polymers
  • Hydroxyl Radical
  • caprolactone