Evaluation of three commercial broth media for pigment detection and identification of a group B Streptococcus (Streptococcus agalactiae)

Maria da Glória Carvalho; Richard Facklam; Delois Jackson; Bernard Beall; Lesley McGee

doi:10.1128/JCM.01374-09

Evaluation of three commercial broth media for pigment detection and identification of a group B Streptococcus (Streptococcus agalactiae)

J Clin Microbiol. 2009 Dec;47(12):4161-3. doi: 10.1128/JCM.01374-09. Epub 2009 Oct 7.

Authors

Maria da Glória Carvalho¹, Richard Facklam, Delois Jackson, Bernard Beall, Lesley McGee

Affiliation

¹ Streptococcus Laboratory, Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

Abstract

Detection of group B Streptococcus (GBS) strains at various bacterial concentrations was evaluated using three pigment-producing broth media. At 10(3) CFU/ml, StrepB carrot broth (SBCB), Granada instant liquid biphasic (IGLB), and Northeast Laboratory GBS screening medium (NEL-GBS) showed 100% detection, but at the lower bacterial counts, SBCB and IGLB were more sensitive than NEL-GBS after 24 h.

Publication types

Evaluation Study

MeSH terms

Bacteriological Techniques / methods
Colony Count, Microbial
Culture Media*
Humans
Pigments, Biological / metabolism*
Reagent Kits, Diagnostic*
Sensitivity and Specificity
Streptococcal Infections* / diagnosis
Streptococcal Infections* / microbiology
Streptococcus agalactiae* / classification
Streptococcus agalactiae* / metabolism

Substances

Culture Media
Pigments, Biological
Reagent Kits, Diagnostic