Abstract
The synthesis is reported of a series of glutamyl peptide analogs of the model substrate H-Ser-Glu-Glu-Glu-Glu-Glu-OH of casein kinase-2 (CK-2). A convenient HPLC method for the separation of slightly different acidic peptides is also reported. The site specificity of yeast casein kinase-2 (Y-CK2) is examined with the aid of synthesized peptide substrates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Casein Kinases
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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Glutamates
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Glutamic Acid
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Indicators and Reagents
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Molecular Sequence Data
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Oligopeptides / chemical synthesis*
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Oligopeptides / isolation & purification
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Oligopeptides / metabolism
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Protein Kinases / metabolism*
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Saccharomyces cerevisiae / enzymology*
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Substrate Specificity
Substances
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Glutamates
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Indicators and Reagents
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Oligopeptides
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Glutamic Acid
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Protein Kinases
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Casein Kinases