IspG enzyme activity in the deoxyxylulose phosphate pathway: roles of the iron-sulfur cluster

Biochemistry. 2009 Nov 10;48(44):10483-5. doi: 10.1021/bi901519q.

Abstract

IspG is a [4Fe-4S] cluster-containing protein, and the [4Fe-4S](+) species is proposed to be the catalytically relevant species. However, attempts reported in the literature failed to detect the [4Fe-4S](+) species. In this study, using a potent reduction system, we have successfully detected the [4Fe-4S](+) species with X-band EPR spectroscopy. In addition, we have improved the Escherichia coli IspG activity to 550 nmol min(-1) mg(-1), which is approximately 20-fold greater than that of the NADPH-Fpr-FldA system in the literature.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biocatalysis
  • Electron Spin Resonance Spectroscopy / methods
  • Iron-Sulfur Proteins / chemistry
  • Iron-Sulfur Proteins / metabolism
  • Iron-Sulfur Proteins / physiology*
  • Nuclear Magnetic Resonance, Biomolecular
  • Oxidation-Reduction
  • Protein Conformation
  • Xylose / analogs & derivatives*
  • Xylose / metabolism

Substances

  • Iron-Sulfur Proteins
  • deoxyxylulose phosphate
  • Xylose