OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice

Mol Plant. 2008 May;1(3):446-58. doi: 10.1093/mp/ssn024.

Abstract

The rice Xa21 gene, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), encodes a receptor-like kinase. Few components involved in transducing the Xa21-mediated defense response have yet been identified. Here, we report that XA21 binds to a WRKY transcription factor, called OsWRKY62. The OsWRKY62 gene encodes two splice variants (OsWRKY62.1 and OsWRKY62.2). OsWRKY62.1:smGFP2 and OsWRKY62.2:smGFP2 fusion proteins partially localize to the nucleus. Transgenic plants overexpressing OsWRKY62.1 are compromised in basal defense and Xa21-mediated resistance to Xoo. Furthermore, overexpression of OsWRKY62.1 suppresses the activation of defense-related genes. These results imply that OsWRKY62 functions as a negative regulator of innate immunity in rice, and serves as a critical mediator of both basal and race-specific defense responses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation, Plant*
  • Genes, Plant
  • Genome, Plant
  • Immunity, Innate
  • Molecular Sequence Data
  • Oryza / genetics*
  • Oryza / microbiology
  • Phylogeny
  • Plant Diseases / microbiology*
  • Plant Diseases / prevention & control*
  • Plant Leaves / microbiology
  • RNA, Messenger / genetics
  • RNA, Plant / genetics
  • Recombinant Fusion Proteins / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors / genetics*
  • Xanthomonas

Substances

  • DNA-Binding Proteins
  • RNA, Messenger
  • RNA, Plant
  • Recombinant Fusion Proteins
  • Transcription Factors

Associated data

  • GENBANK/AAR37421
  • GENBANK/AAX20040
  • GENBANK/AAX76840
  • GENBANK/ABC26917
  • GENBANK/ABI23959
  • GENBANK/ABK55608
  • GENBANK/AF204925
  • GENBANK/BAA24277
  • GENBANK/BAA87058
  • GENBANK/BAD03969
  • GENBANK/CAA88331
  • GENBANK/CAD60651
  • GENBANK/CAH68818
  • GENBANK/DAA05093
  • GENBANK/DAA05127
  • GENBANK/DAA05136
  • GENBANK/DAA05141
  • RefSeq/NP_001063185
  • RefSeq/NP_001066995
  • RefSeq/NP_178199
  • RefSeq/NP_180072
  • RefSeq/NP_194086
  • RefSeq/NP_567882