Taurine reduces inflammatory responses after spinal cord injury

J Neurotrauma. 2010 Feb;27(2):403-10. doi: 10.1089/neu.2009.1044.

Abstract

Taurine has multiple functions in the central nervous system (CNS), serving as an osmoregulator, antioxidant, inhibitory neuromodulator, and regulator of intracellular Ca(2+) flux. Since the role of taurine in traumatic spinal cord injury (SCI) is not fully understood, the present study was conducted with C57 black/6 mice (18-20 g) who underwent severe SCI at the Th-8 level using a weight compression device. Taurine was injected intraperitoneally at doses of 25, 80, 250, and 800 mg/kg within 30 min after SCI. Controls were injected with saline. The contusional cord segments were removed 6 h after SCI, and concentrations of interleukin-6 (IL-6) and myeloperoxidase (MPO) were measured using ELISA kits. Phosphorylation of STAT3, which is activated by IL-6, and expression of inducible cyclooxygenase-2 (COX-2) were also compared between the taurine treatment group (250 mg/kg) and the control group by Western blot analysis. Morphological changes were evaluated with H&E-stained sections. Taurine significantly decreased IL-6 and MPO levels in a dose-dependent manner, significantly reducing the phosphorylation of STAT3 and expression of COX-2 after SCI compared to controls. A reduced accumulation of neutrophils, especially in the subarachnoid spaces, and secondary degenerative changes in gray matter were also noted, and motor disturbances were significantly attenuated with taurine treatment (250 mg/kg). These findings indicate that taurine has anti-inflammatory effects against SCI, and may play a neuroprotective role against secondary damage, and thus it may have therapeutic potential.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Blotting, Western
  • Cyclooxygenase 2 / biosynthesis
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Inflammation / drug therapy*
  • Inflammation / etiology
  • Interleukin-6 / biosynthesis
  • Mice
  • Mice, Inbred C57BL
  • Neutrophil Infiltration / drug effects
  • Peroxidase / biosynthesis
  • Phosphorylation
  • STAT3 Transcription Factor / metabolism
  • Spinal Cord Injuries / complications
  • Spinal Cord Injuries / drug therapy*
  • Spinal Cord Injuries / pathology
  • Taurine / pharmacology*

Substances

  • Anti-Inflammatory Agents
  • Interleukin-6
  • STAT3 Transcription Factor
  • Taurine
  • Peroxidase
  • Cyclooxygenase 2