Abstract
Twenty Nocardia spp. isolated from ocular infections were identified by 16S rRNA gene sequencing and susceptibility was determined using the E-test (AB Biodisk, Sweden). Species distribution among the 20 isolates was as follows: Nocardia levis (n = 7), Nocardia farcinica (n = 3), Nocardia abscessus (n = 2), Nocardia brasiliensis (n = 2), Nocardia amamiensis (n = 2), Nocardia puris (n = 1), Nocardia beijingensis (n = 1), Nocardia otitidiscaviarum (n = 1) and Nocardia thailandica (n = 1). All isolates were sensitive to amikacin. Eighteen (90%) isolates were sensitive to tobramycin, 11 (55%) to ciprofloxacin and gatifloxacin, and seven (35%) to azithromycin and clarithromycin. Molecular methods are useful for the identification and for the detection of Nocardia species that have not so far been reported in human infections.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Cluster Analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Eye Infections / microbiology*
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Nocardia / classification*
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Nocardia / drug effects*
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Nocardia / isolation & purification
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Nocardia Infections / microbiology*
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Sweden
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/GQ340672
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GENBANK/GQ340673
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GENBANK/GQ340674
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GENBANK/GQ340675
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GENBANK/GQ340676
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GENBANK/GQ340677
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GENBANK/GQ340678
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GENBANK/GQ340679
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GENBANK/GQ340680
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GENBANK/GQ340681
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GENBANK/GQ340682
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GENBANK/GQ340683
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GENBANK/GQ340684
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GENBANK/GQ340685
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GENBANK/GQ340686
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GENBANK/GQ340687
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GENBANK/GQ340688
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GENBANK/GQ340689
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GENBANK/GQ340690
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GENBANK/GQ340691