Abstract
A 3D load-providing culture system is presented, which enables human cancellous bone explants to be studied ex vivo. Bone integrity and activity was demonstrated during 2 to 3 weeks of culture. Viable osteocytes and bone specific markers could be identified. Culture conditions were improved using a serum free medium containing TGF-beta3. Therefore this system gives the opportunity to study bone biology, hormonal effects as well as biomaterial interactions ex vivo prior to animal studies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bone Diseases / metabolism
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Bone and Bones / cytology
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Bone and Bones / physiology*
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods
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Cell Survival
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Cells, Cultured / cytology
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Cells, Cultured / metabolism
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Culture Media
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Humans
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Osteoblasts / cytology
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Osteoclasts / cytology
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Osteocytes / cytology
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Osteocytes / drug effects
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Transforming Growth Factor beta3 / pharmacology
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Weight-Bearing
Substances
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Culture Media
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Transforming Growth Factor beta3