A methicillin-resistant (MR) Staphylococcus epidermidis strain was isolated from a saddle horse affected by osteolysis. MR coagulase-negative staphylococci (MRCNS) were isolated from 11 of 14 (78.8%) horses housed in the same riding club. By typing of the SCCmec region, almost the strains displayed a non typeable (NT) pattern and possessed the ccr type 2. Altogether, the high prevalence of MRCNS and the detection of NT SCCmec types support the hypothesis that horses may represent a reservoir of MRCNS for humans and that equine MRCNS may act as potential source of resistance genes for other staphylococci.