Abstract
The design of artificial extracellular matrices (ECM) has attracted much attention in tissue engineering and regenerative medicine as well as in molecular biology research. A recombinant hepatocyte growth factor (HGF), fused to an immunoglobulin G (IgG) Fc region (abbreviated as AeHGF-Fc) was constructed and confirmed by Western blot assay. Almost similar amounts of HepG2 cells adhered to AeHGF-Fc-coated surface compared to collagen-coated one with large morphological changes. Immobilized AeHGF-Fc continuously activated Akt in HepG2 cells whereas Akt activation induced by soluble HGF rapidly decreased with time, indicating that immobilized AeHGF-Fc follows different signal transduction pathways compared to soluble HGF.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomimetic Materials / chemistry*
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Biomimetic Materials / metabolism*
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Cell Adhesion
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Cell Culture Techniques / methods
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Cell Proliferation
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Extracellular Matrix / chemistry*
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Extracellular Matrix / metabolism*
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Hep G2 Cells
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Hepatocyte Growth Factor / chemistry*
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Hepatocyte Growth Factor / genetics
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Hepatocyte Growth Factor / metabolism*
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Humans
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Immunoglobulin G / chemistry*
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Immunoglobulin G / genetics
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Immunoglobulin G / metabolism*
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Materials Testing
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Receptors, Fc / chemistry
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Receptors, Fc / genetics
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Receptors, Fc / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Tissue Engineering / methods
Substances
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Immunoglobulin G
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Receptors, Fc
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Recombinant Fusion Proteins
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Hepatocyte Growth Factor