Abstract
We compared PCR to conventional culture for the detection of vancomycin-resistant enterococci (VRE) in 30,835 rectal samples over a 3-year period. The positive and negative predictive values of vanB PCR were 1.42% and 99.9%, respectively. A positive vanB result by PCR is poorly predictive and necessitates culture for differentiation of VRE-positive and -negative individuals.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Bacterial Proteins / genetics*
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Bacteriological Techniques
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Carbon-Oxygen Ligases / genetics*
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Culture Media*
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification
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Enterococcus / classification
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Enterococcus / drug effects*
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Enterococcus / genetics
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Enterococcus / isolation & purification
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Humans
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Mass Screening / methods*
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Polymerase Chain Reaction / methods*
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Predictive Value of Tests
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Sensitivity and Specificity
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Vancomycin Resistance* / genetics
Substances
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Bacterial Proteins
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Culture Media
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DNA, Bacterial
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VanA ligase, Bacteria
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VanB protein, Enterococcus
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Carbon-Oxygen Ligases