Abstract
We investigated anti-invasive effects of the anthocyanins from fruits of Vitis coignetiae Pulliat (known as meoru in Korea) on human hepatoma Hep3B cells. The anthocyanins inhibited cell invasion in a dose-dependent manner as measured by Matrigel (BD Biosciences, San Jose, CA, USA) invasion assays. They also inhibited expression of matrix metalloproteinase (MMP)-2 and MMP-9 and activation of nuclear factor kappaB (NF-kappaB) stimulated by tumor necrosis factor alpha. Taken together, the results of this study indicate that the anthocyanins isolated from fruits of V. coignetiae Pulliat have anti-invasive effects on human hepatoma Hep3B cells and inhibit MMP-2 and MMP-9 gene expression at least in part through the inhibition of NF-kappaB activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anthocyanins / isolation & purification
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Anthocyanins / pharmacology
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Anthocyanins / therapeutic use*
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Antineoplastic Agents, Phytogenic / therapeutic use*
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Carcinoma, Hepatocellular / drug therapy*
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Carcinoma, Hepatocellular / pathology
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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Fruit
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Humans
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Liver Neoplasms / drug therapy*
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Liver Neoplasms / pathology
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Matrix Metalloproteinase Inhibitors
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NF-kappa B / antagonists & inhibitors
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Neoplasm Invasiveness
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Phytotherapy
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use*
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Vitis / chemistry*
Substances
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Anthocyanins
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Antineoplastic Agents, Phytogenic
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Matrix Metalloproteinase Inhibitors
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NF-kappa B
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Plant Extracts