Abstract
The reported inability to establish long-term T-cell lines from the blood of cutaneous T-cell lymphoma patients with circulating neoplastic T cells has hindered the development of an in vitro system to investigate Sézary syndrome. We have established a rapidly proliferating T-cell line from the peripheral blood of a patient with Sézary syndrome, which expresses a mature helper T-cell phenotype and contains cytogenetic abnormalities and T-cell receptor gene rearrangements identical to those in the patient's blood. The method of establishment and characteristics of this line are described.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aged
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Antibodies, Monoclonal
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Antigens, CD / analysis
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Antigens, Surface / analysis
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CD4 Antigens / analysis*
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Cell Line
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Culture Techniques / methods
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DNA Probes
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DNA Replication / drug effects
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Female
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Humans
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Interleukin-2 / pharmacology
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Lymphocyte Activation
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Phenotype
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Sezary Syndrome / immunology*
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Skin Neoplasms / immunology*
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology*
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T-Lymphocytes / ultrastructure
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Surface
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CD4 Antigens
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DNA Probes
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DNA, Neoplasm
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Interleukin-2