[High prevalence of community- and hospital-acquired infections of methicillin-resistant Staphylococcus aureus containing Panton-Valentine leukocidin gene in Algiers]

K Antri; N Rouzic; I Boubekri; O Dauwalder; A Beloufa; H Ziane; F Djennane; M Neggazi; B Benhabyles; M Bes; M Tazir; J Etienne; N Ramdani-Bouguessa

doi:10.1016/j.patbio.2009.07.017

[High prevalence of community- and hospital-acquired infections of methicillin-resistant Staphylococcus aureus containing Panton-Valentine leukocidin gene in Algiers]

Pathol Biol (Paris). 2010 Apr;58(2):e15-20. doi: 10.1016/j.patbio.2009.07.017. Epub 2009 Oct 28.

[Article in French]

Authors

K Antri¹, N Rouzic, I Boubekri, O Dauwalder, A Beloufa, H Ziane, F Djennane, M Neggazi, B Benhabyles, M Bes, M Tazir, J Etienne, N Ramdani-Bouguessa

Affiliation

¹ Service de microbiologie, centre hospitalo-universitaire Mustapha-Bacha, place du Premier-Mai, 16000 Alger, Algérie.

PMID: 19875247
DOI: 10.1016/j.patbio.2009.07.017

Abstract

Objectives: To determine the prevalence of community acquired and hospital methicillin-resistant Staphylococcus aureus (S. aureus) infections and the Panton-Valentine leukocidin.

Patients and methods: Seven hundred S. aureus strains were collected during 21 months period in Mustapha Bacha hospital. Bacterial identification was based on standard methods and susceptibilities were tested by disk diffusion method. Molecular study (toxins, mecA gene and agr alleles) were determined for 221 S. aureus isolates by multiplex PCR.

Results: The global MRSA prevalence was 42 %, 35 % in the community and 49 % in hospital setting. The frequency of strains containing PVL genes (PVL+) was 36 %, their molecular profile was: agr3, mecA+, etd, edin, which correspond to the C-MRSA major ST80 clone in Europe and the Maghreb. The H-MRSA-PVL+ were multidrug resistant. Among the MSSA, 13 strains contained the tst gene and five contained the exfoliatine genes ETA and ETB.

Conclusion: Our results show a high rate of MRSA-PVL+ in the community and the hospital setting. The H-MRSA-PVL+ were multidrug resistant complicating their antibiotic treatment options.

Publication types

Comparative Study

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Algeria / epidemiology
Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Bacterial Proteins / genetics
Bacterial Toxins / genetics*
Child
Child, Preschool
Community-Acquired Infections / epidemiology
Community-Acquired Infections / microbiology*
Cross Infection / epidemiology
Cross Infection / microbiology*
Drug Resistance, Multiple, Bacterial / genetics
Enterotoxins / genetics
Exfoliatins / genetics
Exotoxins / genetics*
Female
Genes, Bacterial
Hospitals, University / statistics & numerical data
Humans
Infant
Leukocidins / genetics*
Male
Methicillin Resistance / genetics
Methicillin-Resistant Staphylococcus aureus / drug effects
Methicillin-Resistant Staphylococcus aureus / genetics
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Middle Aged
Organ Specificity
Penicillin-Binding Proteins
Prevalence
Staphylococcal Infections / epidemiology
Staphylococcal Infections / microbiology*
Superantigens / genetics
Young Adult

Substances

Anti-Bacterial Agents
Bacterial Proteins
Bacterial Toxins
Enterotoxins
Exfoliatins
Exotoxins
Leukocidins
Panton-Valentine leukocidin
Penicillin-Binding Proteins
Superantigens
enterotoxin F, Staphylococcal
mecA protein, Staphylococcus aureus