Phoma herbarum as a new gibberellin-producing and plant growth-promoting fungus

J Microbiol Biotechnol. 2009 Oct;19(10):1244-9.

Abstract

Endophytic fungi are known for the production of valuable metabolites, but information on the gibberellin production capacity of this group is limited. We isolated 9 endophytic fungi from the roots of salt-stressed soybean plants and screened them on waito-c rice, in order to identify plant growth promoting fungal strains. The fungal isolate TK- 2-4 gave maximum plant length (20.35 cm) promotion in comparison with wild-type Gibberella fujikuroi (19.5 cm). In a separate experiment, bioassay of TK-2-4 promoted plant length and biomass of soybean cultivar Taegwangkong. The TK-2-4 culture filtrate was analyzed for the presence of gibberellins, and it was found that all physiologically active gibberellins, especially GA(4) and GA(7), were present in higher amounts (GA(1), 0.11 ng/ml; GA(3), 2.91 ng/ml; GA(4), 3.21 ng/ml; and GA(7), 1.4 ng/ml) in conjunction with physiologically inactive GA(9) (0.05 ng/ml), GA(12) (0.23 ng/ ml), GA(15) (0.42 ng/ml), GA(19) (0.53 ng/ml), and GA(20) (0.06 ng/ml). The fungal isolate TK-2-4 was later identified as a new strain of Phoma herbarum, through the phylogenetic analysis of 28S rDNA sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / classification
  • Ascomycota / genetics
  • Ascomycota / isolation & purification*
  • Ascomycota / metabolism
  • Biomass
  • Gibberellins / metabolism*
  • Glycine max / growth & development
  • Glycine max / microbiology*
  • Molecular Sequence Data
  • Oryza / growth & development*
  • Oryza / microbiology
  • Phylogeny
  • Plant Roots / growth & development
  • Plant Roots / microbiology

Substances

  • Gibberellins