[Conditions for extraction of ergosterol from fruits of Ganoderma lucidum using supercritical fluid extration]

Zhongguo Zhong Yao Za Zhi. 2009 Jul;34(14):1783-5.
[Article in Chinese]

Abstract

Objective: To study the feasibility of supercritical fluid extraction (SFE) for extraction of ergosterols from the fruits of Ganoderma lucidum.

Method: Optimum extraction conditions were studied by orthogonal tests, the ergosterol were analyzed by RP-HPLC.

Result: The optimal extraction conditions were pressure as 20 MPa, temperature at 45 degrees C, using ethanol as modifier carrier at the volume of 1 mL x g(-1), and 1.5 hour of extraction time.

Conclusion: SFE has the superiority of adjustable polarity, and has the ability of extracting ergosterol from fruits of Ganoderma lucidum.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Supercritical Fluid / methods*
  • Ergosterol / analysis
  • Ergosterol / isolation & purification*
  • Fruit / chemistry
  • Plant Extracts / analysis
  • Plant Extracts / isolation & purification*
  • Reishi / chemistry*

Substances

  • Plant Extracts
  • Ergosterol