Objective: To introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots.
Method: Hairy roots were obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC.
Result: The best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-days co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The E1 was the excellent roots.
Conclusion: Hairy root of E. ulmoides will be useful for the production of active components in aucubin.