[Effect of Santalum album on tissue distribution of danshensu in rabbits by HPLC]

Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(15):1968-70.
[Article in Chinese]

Abstract

Objective: To investigate the influence of Shi herb (Santalum album, SA) to the tissue distribution of danshensu (DSS) which is the main hydrosoluble component of Jun herb (Salvia miltiorrhiza, SM) in rabbits by HPLC.

Method: Rabbits were oral administrated decoction of SM and SM-SA, respectively. Perchloric acid (10%) was used to precipitate the tissue samples of rabbits heart, brain, liver, kidney, acetic ether was used to extracte supernatant, and the internal standard was p-hydroxybenzoic acid. The content of DSS of SM in tissues was detected.

Result: The content of DSS reached the highest point close to 50 min in the mentioned tissues. Before and after co-administration, the sequences of average concentration of DSS in tissues were C(kidney) > C(heart) > C(brain) > C(liver) and C(kidney) > C(liver) > C(brain) > C(heart) respectively. Compared with SM administrated singly, the content of DSS in every tissues of co-administration was higher.

Conclusion: In Danshenyin Formulae, SA can increase concentration of DSS in target tissues significantly, and therefore therapeutic effect of SM for cardiovascular disease is raised.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / drug effects
  • Chromatography, High Pressure Liquid
  • Drugs, Chinese Herbal / administration & dosage
  • Drugs, Chinese Herbal / analysis
  • Drugs, Chinese Herbal / pharmacokinetics*
  • Heart / drug effects
  • Kidney / drug effects
  • Lactates / administration & dosage
  • Lactates / analysis
  • Lactates / pharmacokinetics*
  • Liver / drug effects
  • Rabbits
  • Santalum / chemistry*

Substances

  • Drugs, Chinese Herbal
  • Lactates
  • 3,4-dihydroxyphenyllactic acid