Fabrication of cellular polycaprolactone films for cell culture

Colloids Surf B Biointerfaces. 2010 Mar 1;76(1):38-43. doi: 10.1016/j.colsurfb.2009.10.006. Epub 2009 Oct 14.

Abstract

Hierarchical cellular structures consisting of multilayers of ordered pores were created on a poly-epsilon-caprolactone (PCL) film by introducing a colloidal crystal mask/template in thermomechanical lithography. The surface characteristics were measured by scanning electron microscopy, atomic force microscopy, water contact angle and analyzed by Fourier Transform (FT). The resultant PCL films present a metastable superhydrophobicity. Their water contact angles were initially 150 degrees and then declined with time eventually to 115-120 degrees . In vitro culture of human fibroblasts found that the cells could spread on the cellular PCL surface as normal, but showed higher viability compared with the control cells on a flat substrate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion
  • Cell Culture Techniques*
  • Colloids / chemistry
  • Fibroblasts / chemistry
  • Glass / chemistry
  • Humans
  • Microscopy, Electron, Scanning
  • Polyesters / chemistry*
  • Surface Properties

Substances

  • Colloids
  • Polyesters
  • polycaprolactone