Abstract
In the present study, we cloned and characterized a zebrafish follicle-stimulating hormone (zfFSH)beta promoter with deletion fragments transfected into a tilapia ovary (TO2) cell line, and demonstrated that the zfFSHbeta promoter responded to 6h of gonadotropin-releasing hormone (GnRH) treatment by activating calcium influx and protein kinase C (PKC), but after 24h, GnRH induction was generated by activation of extracellular-regulated kinase (ERK)1/2 and repression by PKC. Furthermore, to study the promoter-specific expression, we constructed a series of FSHbeta (4.0-, 3.0-, 2.0-, and 1.0-kb) promoter-driven green fluorescent protein (GFP) fragments encoding the GFP complementary DNA transgene which was microinjected into zebrafish embryos. Morphological studies of transgenic zebrafish indicated that the FSHbeta promoter-driven GFP transcripts appeared in the heart, skin, and vertebrae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Bioreactors
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Calcium / pharmacology
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Cell Line
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Cloning, Molecular
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Embryo, Nonmammalian / metabolism
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Enzyme Activation
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Female
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Fertilization
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Follicle Stimulating Hormone, beta Subunit / genetics*
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Follicle Stimulating Hormone, beta Subunit / metabolism
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Gene Expression Regulation / drug effects
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Gonadotropin-Releasing Hormone / metabolism
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3 / metabolism
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Ovary / cytology
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Ovary / drug effects
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Ovary / metabolism
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Ovum / physiology
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Pituitary Gland / metabolism
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Promoter Regions, Genetic / genetics*
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Protein Kinase C / metabolism
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Signal Transduction
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Time Factors
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Zebrafish / embryology
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Zebrafish / genetics*
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Zebrafish / physiology
Substances
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Follicle Stimulating Hormone, beta Subunit
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Gonadotropin-Releasing Hormone
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Protein Kinase C
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Calcium