Appositional bone formation by OCP-collagen composite

J Dent Res. 2009 Dec;88(12):1107-12. doi: 10.1177/0022034509351378. Epub 2009 Nov 6.

Abstract

Synthetic octacalcium phosphate (OCP) has been shown to enhance bone formation and to biodegrade if implanted into bone defects. Here, we hypothesized that an OCP-atelocollagen complex (OCP/Col) is biodegradable and can induce bone formation in a thickness-dependent manner when implanted into the calvaria. OCP/Col disks (diameter, 9 mm; thickness, 1 or 3 mm) were implanted into a subperiosteal pocket in the calvaria of 12-week-old Wistar rats for 4, 8, and 12 weeks and subsequent bone formation was monitored. X-ray diffraction analysis and Fourier transform infrared spectroscopy showed that OCP in the OCP/Col implants was converted into a carbonate-rich apatite after 4 weeks. Although thinner disks tended to be replaced by new bone, thicker disks were progressively resorbed by osteoclast-like cells until 12 weeks, possibly via the increased mechanical load in the subperiosteal pocket. Therefore, OCP/Col can increase appositional intra-membranous bone formation if the appropriate size of the implant is applied.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants
  • Acid Phosphatase / analysis
  • Animals
  • Apatites / chemistry
  • Biomarkers / analysis
  • Bone Substitutes / chemistry
  • Bone Substitutes / therapeutic use*
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / therapeutic use*
  • Cathepsin K / analysis
  • Collagen / chemistry
  • Collagen / therapeutic use*
  • Drug Carriers
  • Isoenzymes / analysis
  • Male
  • Osteoclasts / pathology
  • Osteogenesis / physiology*
  • Periosteum / pathology
  • Periosteum / surgery
  • Prosthesis Design
  • Rats
  • Rats, Wistar
  • Skull / pathology
  • Skull / surgery
  • Spectroscopy, Fourier Transform Infrared
  • Stress, Mechanical
  • Tartrate-Resistant Acid Phosphatase
  • Time Factors
  • X-Ray Diffraction

Substances

  • Apatites
  • Biomarkers
  • Bone Substitutes
  • Calcium Phosphates
  • Drug Carriers
  • Isoenzymes
  • atelocollagen
  • octacalcium phosphate
  • carboapatite
  • Collagen
  • Acid Phosphatase
  • Tartrate-Resistant Acid Phosphatase
  • Cathepsin K