Use of oligonucleotides conjugated to gold nanoparticles and streptavidin for amplification of optical biosensor signal during detection of telomeric repeats

S P Rad'ko; S A Voronina; A V Gromov; O V Gnedenko; N V Bodoev; A S Ivanov; K N Yarygin

doi:10.1007/s10517-009-0616-x

Use of oligonucleotides conjugated to gold nanoparticles and streptavidin for amplification of optical biosensor signal during detection of telomeric repeats

Bull Exp Biol Med. 2009 Jun;147(6):746-9. doi: 10.1007/s10517-009-0616-x.

Authors

S P Rad'ko¹, S A Voronina, A V Gromov, O V Gnedenko, N V Bodoev, A S Ivanov, K N Yarygin

Affiliation

¹ V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.

PMID: 19902073
DOI: 10.1007/s10517-009-0616-x

Abstract

Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied by the surface plasmon resonance method. Conjugation of the probe with streptavidin and gold nanoparticles was shown to amplify the signal at similar concentrations of this probe (by 60 and 300 times, respectively). Nanoparticles can be used for biosensor signal amplification in studying the telomerase activity of malignant cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Gold / chemistry*
Metal Nanoparticles / chemistry*
Oligonucleotide Probes / chemistry*
Streptavidin / chemistry*
Telomerase / metabolism
Telomere / chemistry*
Telomere / genetics

Substances

Oligonucleotide Probes
Gold
Streptavidin
Telomerase