Synthesis, characterization, interaction with DNA and cytotoxicity in vitro of the complexes [M(dmphen)(CO3)].H2O [M=Pt(II), Pd(II)]

En-jun Gao; Lei Wang; Ming-chang Zhu; Lei Liu; Wan-zhong Zhang

doi:10.1016/j.ejmech.2009.10.014

Synthesis, characterization, interaction with DNA and cytotoxicity in vitro of the complexes [M(dmphen)(CO3)].H2O [M=Pt(II), Pd(II)]

Eur J Med Chem. 2010 Jan;45(1):311-6. doi: 10.1016/j.ejmech.2009.10.014. Epub 2009 Oct 22.

Authors

En-jun Gao¹, Lei Wang, Ming-chang Zhu, Lei Liu, Wan-zhong Zhang

Affiliation

¹ Laboratory of Coordination Chemistry, Shenyang Institute of Chemical Technology, Shenyang 110142, China. [email protected]

PMID: 19913954
DOI: 10.1016/j.ejmech.2009.10.014

Abstract

The complexes [Pt(dmphen)CO3].H2O (1), [Pd(dmphen)CO3].H2O (2) (dmphen is 2,9-dimethyl-1,10-phenanthroline) have been synthesized and characterized. The binding of the complexes with FS-DNA was investigated by UV spectrum and fluorescence spectrum, showing that the complexes have the ability of interaction with DNA of intercalative mode. The intrinsic binding constant K of the complexes with FS-DNA is 1.8 x 10(5) M(-1) (1) and 1.6 x 10(4) M(-1) (2), respectively. Gel electrophoresis assay demonstrated the ability of the complexes to cleave the pBR 322 plasmid DNA. Evaluation of cytotoxic activity of the complexes against four different cancer cell lines proved that the complexes exhibited cytotoxic specificity and significant cancer cell inhibitory rate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Proliferation / drug effects
DNA / chemistry
DNA / metabolism*
Humans
Models, Molecular
Nucleic Acid Conformation
Organoplatinum Compounds / chemical synthesis
Organoplatinum Compounds / chemistry
Organoplatinum Compounds / metabolism*
Organoplatinum Compounds / pharmacology*
Palladium / chemistry*
Spectrum Analysis
Water / chemistry

Substances

Organoplatinum Compounds
Water
Palladium
DNA