Effects of dexmedetomidine pretreatment on bupivacaine cardiotoxicity in rats

Reg Anesth Pain Med. 2009 Nov-Dec;34(6):565-8. doi: 10.1097/aap.0b013e3181bfbe35.

Abstract

Background and objective: We evaluated the effects of dexmedetomidine pretreatment on bupivacaine cardiotoxicity in anesthetized rats.

Methods: Sixteen Wistar-Albino male rats (300-400 g) were anesthetized with ketamine. Electrocardiographic and invasive blood pressure monitoring were performed, and the results were continuously recorded. The rats were randomized into 2 groups. In group D, rats were pretreated with intravenous dexmedetomidine at a dose of 10 Kg/kg (n = 8), whereas in group S, rats were pretreated with intravenous saline (n = 8). Fifteen minutes later, bupivacaine was infused at a rate of 3 mg/kg per minute until cardiac asystole occurred. The timing of specific cardiotoxic events (a 25%, 50%, and 75% reductions of mean arterial pressure and heart rate as well as occurrence of the first arrhythmia and asystole) was recorded.

Results: Dexmedetomidine pretreatment reduced the heart rates and mean arterial pressures of the rats who received it (P G 0.05). Dexmedetomidine pretreatment before bupivacaine administration also significantly increased the time to the 25%, 50%, and 75% reductions in mean arterial pressure and the time to the 25% and 50% reductions in heart rate (P G 0.05). In addition, dexmedetomidine significantly increased the time to first arrhythmia and time to asystole (P G 0.05) in the rats who received it before receiving bupivacaine.

Conclusions: Dexmedetomidine pretreatment delays the effects of bupivacaine cardiotoxicity.

MeSH terms

  • Adrenergic alpha-Agonists / administration & dosage
  • Adrenergic alpha-Agonists / pharmacology*
  • Anesthetics, Local / adverse effects*
  • Animals
  • Arrhythmias, Cardiac / chemically induced*
  • Blood Pressure / drug effects
  • Bupivacaine / adverse effects*
  • Dexmedetomidine / administration & dosage
  • Dexmedetomidine / pharmacology*
  • Electrocardiography / drug effects
  • Heart / drug effects*
  • Heart Rate / drug effects
  • Male
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Treatment Outcome

Substances

  • Adrenergic alpha-Agonists
  • Anesthetics, Local
  • Dexmedetomidine
  • Bupivacaine