Abstract
In this contribution, a biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel system was successfully prepared for basic fibroblastic growth factor (bFGF) antigen delivery. bFGF encapsulated PECE hydrogel system (bFGF-hydrogel) is an injectable free-flowing sol at ambient temperature, and forms a non-flowing gel at physiological temperature acting as antigen depot. Furthermore, the cytotoxicity results showed that the PECE hydrogel could be regarded as a safe carrier, and bFGF could be released from the hydrogel system in an extended period in vitro. Otherwise, the immunogenicity of bFGF was improved significantly after encapsulated into the hydrogel. Strong humoral immunity created by bFGF-hydrogel was maintained for more than 14 weeks. Therefore, the prepared bFGF loaded PECE hydrogel might have great potential as a novel vaccine adjuvant for protein antigen.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / blood
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Antigens* / administration & dosage
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Antigens* / immunology
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Biocompatible Materials
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Delayed-Action Preparations
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Drug Delivery Systems*
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Female
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Fibroblast Growth Factor 2* / administration & dosage
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Fibroblast Growth Factor 2* / immunology
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Hot Temperature
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Humans
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Hydrogel, Polyethylene Glycol Dimethacrylate* / administration & dosage
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Hydrogel, Polyethylene Glycol Dimethacrylate* / toxicity
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Immunity, Humoral
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Injections
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L Cells
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Mice
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Mice, Inbred BALB C
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Polyesters* / administration & dosage
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Polyesters* / toxicity
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Polyethylene Glycols* / administration & dosage
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Polyethylene Glycols* / toxicity
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Vaccines / administration & dosage
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Vaccines / immunology
Substances
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Antibodies
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Antigens
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Biocompatible Materials
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Delayed-Action Preparations
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Polyesters
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Vaccines
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poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol)
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Fibroblast Growth Factor 2
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Polyethylene Glycols