A new sol-gel silica nanovehicle preparation for photodynamic therapy in vitro

Int J Pharm. 2010 Feb 15;386(1-2):131-7. doi: 10.1016/j.ijpharm.2009.11.010. Epub 2009 Nov 14.

Abstract

We report a facile silica nanovehicle preparation procedure for hydrophobic drug delivery, which is carried out in water without adding any surfactant and additional catalyst. This strategy includes hydrophobic drug nanopaticle preparation by reprecipitation method and in situ hydrolyzation and polymerization to encapsulate this naoparticle using only N-(beta-amimoethyl)-gamma-aminopropyltriethoxysilane (AETPS). To demonstrate this technique hypocrellin A (HA), a hydrophobic photosensitizing anticancer drug, is embedded into silica nanovehicle using this simple method. The resulting HA encapsulated nanovehicles (HANV) are monodisperse and stable in aqueous solution. Comparative studies with free HA and entrapped HA have demonstrated that the encapsulation effect on the embedded photosensitizer nanoparticle significantly enhances the efficacy of singlet oxygen generation and, thereby, the in vitro photodynamic efficacy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Biological Transport
  • Cell Survival / drug effects
  • Chemical Precipitation
  • Chemistry, Pharmaceutical
  • Drug Carriers*
  • Drug Compounding
  • Drug Stability
  • Gels
  • HeLa Cells
  • Humans
  • Hydrogen Bonding
  • Membrane Potential, Mitochondrial / drug effects
  • Microscopy, Electron, Transmission
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Nanoparticles*
  • Particle Size
  • Perylene / analogs & derivatives*
  • Perylene / chemistry
  • Perylene / metabolism
  • Perylene / pharmacology
  • Perylene / radiation effects
  • Pharmaceutical Vehicles / chemistry*
  • Pharmaceutical Vehicles / toxicity
  • Phenol
  • Photochemotherapy / methods*
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / metabolism
  • Photosensitizing Agents / pharmacology*
  • Photosensitizing Agents / radiation effects
  • Propylamines
  • Quinones / chemistry
  • Quinones / metabolism
  • Quinones / pharmacology*
  • Quinones / radiation effects
  • Reactive Oxygen Species / metabolism
  • Reproducibility of Results
  • Silanes / chemistry*
  • Silanes / toxicity
  • Singlet Oxygen / chemistry
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Technology, Pharmaceutical / methods
  • Time Factors

Substances

  • Drug Carriers
  • Gels
  • Pharmaceutical Vehicles
  • Photosensitizing Agents
  • Propylamines
  • Quinones
  • Reactive Oxygen Species
  • Silanes
  • Singlet Oxygen
  • Phenol
  • Perylene
  • amino-propyl-triethoxysilane
  • hypocrellin A