FEZ1 interacts with CLASP2 and NEK1 through coiled-coil regions and their cellular colocalization suggests centrosomal functions and regulation by PKC

Daniel C F Lanza; Gabriela V Meirelles; Marcos R Alborghetti; Camila H Abrile; Guido Lenz; Jörg Kobarg

doi:10.1007/s11010-009-0317-9

FEZ1 interacts with CLASP2 and NEK1 through coiled-coil regions and their cellular colocalization suggests centrosomal functions and regulation by PKC

Mol Cell Biochem. 2010 May;338(1-2):35-45. doi: 10.1007/s11010-009-0317-9. Epub 2009 Nov 19.

Authors

Daniel C F Lanza¹, Gabriela V Meirelles, Marcos R Alborghetti, Camila H Abrile, Guido Lenz, Jörg Kobarg

Affiliation

¹ Centro de Biologia Molecular Estrutural, Associação Brasileira de Tecnologia de Luz Síncrotron, Rua Giuseppe Máximo Scolfaro, Campinas, SP, Brazil.

PMID: 19924516
DOI: 10.1007/s11010-009-0317-9

Abstract

FEZ1 was initially described as a neuronal protein that influences axonal development and cell polarization. CLASP2 and NEK1 proteins are present in a centrosomal complex and participate in cell cycle and cell division mechanisms, but their functions were always described individually. Here, we report that NEK1 and CLASP2 colocalize with FEZ1 in a perinuclear region in mammalian cells, and observed that coiled-coil interactions occur between FEZ1/CLASP2 and FEZ1/NEK1 in vitro. These three proteins colocalize and interact with endogenous gamma-tubulin. Furthermore, we found that CLASP2 is phosphorylated and interacts with active PKC isoforms, and that FEZ1/CLASP2 colocalization is inhibited by PMA treatment. Our results provide evidence that these three proteins cooperate in centrosomal functions and open new directions for future studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Animals
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cell Line
Cell Nucleus / metabolism
Centrosome / metabolism*
Humans
Isoenzymes / genetics
Isoenzymes / metabolism
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / metabolism*
NIMA-Related Kinase 1
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Protein Binding
Protein Kinase C / genetics
Protein Kinase C / metabolism*
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism*
Protein Structure, Tertiary*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Tubulin / metabolism

Substances

Adaptor Proteins, Signal Transducing
CLASP2 protein, human
Cell Cycle Proteins
FEZ1 protein, human
Isoenzymes
Microtubule-Associated Proteins
Nerve Tissue Proteins
Recombinant Fusion Proteins
Tubulin
NEK1 protein, human
NIMA-Related Kinase 1
Protein Serine-Threonine Kinases
Protein Kinase C