Abstract
Contact of axons with target territories results in the formation of synapses, specific junctional complexes that may represent a final stage of neuronal maturation. Synaptosomal-associated protein 25 (SNAP-25) is a component of particular nerve terminals recently identified in rodent brain. To evaluate the structure and regulation of molecular components of the synapse, we investigated the expression of SNAP-25 in the developing chicken nervous system. Analysis of SNAP-25 cDNA clones demonstrated that the chicken homologue is identical in amino acid sequence to the mouse protein. In chicken retina and neural tube, the onset of SNAP-25 mRNA and protein expression was found to correspond to the time of synaptogenesis. These results suggest that SNAP-25 plays a role in the physiology of mature nerve terminals and that its expression may be regulated by specific cell-cell interactions occurring during synapse formation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / embryology*
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Brain / physiology
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Chick Embryo
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Chickens
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Cloning, Molecular
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Ganglia, Spinal / physiology
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Gene Library
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Immunoblotting
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Immunohistochemistry
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Membrane Proteins*
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Mice
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Molecular Sequence Data
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Nerve Endings / physiology*
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / genetics*
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Neurons / physiology*
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Nucleic Acid Hybridization
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RNA, Messenger / genetics
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Retina / embryology*
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Retina / physiology
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Sequence Homology, Nucleic Acid
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Spinal Cord / embryology*
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Spinal Cord / physiology
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Synaptosomal-Associated Protein 25
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Synaptosomes / physiology*
Substances
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Membrane Proteins
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Nerve Tissue Proteins
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RNA, Messenger
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Snap25 protein, mouse
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Synaptosomal-Associated Protein 25
Associated data
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GENBANK/M57957
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GENBANK/M67501
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GENBANK/M67502
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GENBANK/M67503
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GENBANK/M67504
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GENBANK/M94197
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GENBANK/M94198
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GENBANK/M94199
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GENBANK/M94200
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GENBANK/X53420