A pilot clearwell was used to simulate the chlorine disinfection process with the Bacillus subtilis spores as the target microbe. The effluent of the activated carbon filter tank was radiated by low pressure UV lamp and then used as the influent the pilot clearwell. The impacts of hydraulic characteristics of pilot clearwell on disinfection efficiency of Bacillus subtilis spores was studied under different hydraulic characteristics which was changed by the number of the baffles. Under the conditions of this experiment, the inactivation coefficients of Bacillus subtilis spores with NaC10 as disinfectant which were calculated by Ct10 value were almost same under different hydraulic characteristics, but the inactivation coefficients which were calculated by CT value were very different under different hydraulic characteristics. This verified that it was more reasonable to evaluate the disinfection efficiency by Ct10 value than CT value. When Ct10 value was in the range of 100 - 300 mg x min/L, the inactivation coefficient of Bacillus subtilis spores with NaClO as disinfectant was 0.001 6 L(mg x min), which highly coincided with others' results. When CT value was in the range of 100 - 700 mg x min/L, under the same CT value, the disinfection efficiency of target microbe would be notably enhanced by increasing the number of baffles which would improve the hydraulic characteristics. So the results verified that the disinfection efficiency could be enhanced by improving the hydraulic characteristics of the clearwell.