Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology

J Clin Microbiol. 2010 Feb;48(2):615-6. doi: 10.1128/JCM.01790-09. Epub 2009 Nov 25.

Abstract

To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers / genetics*
  • Enterovirus / genetics
  • Enterovirus / isolation & purification*
  • Enterovirus Infections / diagnosis*
  • Humans
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • DNA Primers