Isolation of cutaneous dendritic cells

Methods Mol Biol. 2010:595:231-3. doi: 10.1007/978-1-60761-421-0_15.

Abstract

Cutaneous dendritic cells represent the first immunological interface with the environment and play a key role in the defense against pathogens that breach the skin. This protocol describes how to isolate cutaneous dendritic cells from mouse ears for flow cytometry analysis and functional assay studies.

MeSH terms

  • Animals
  • Cell Separation / methods*
  • Cells, Cultured
  • Dermis / cytology
  • Epidermal Cells
  • Flow Cytometry
  • Langerhans Cells / cytology*
  • Mice