Abstract
In this study, three acidic polysaccharides (APS-3a, APS-3b and APS-3c) were obtained from Angelica sinensis (Oliv.) Diels. They displayed different structural features and anti-tumor activities. APS-3b and APS-3c significantly inhibited the growth of S180 tumors and increased the life spans of S180 tumor-bearing mice, whereas APS-3a had no significant effect. Further experiments showed that APS-3b and APS-3c could cause a concentration-dependent proliferation of the splenocytes, up-regulate IFN-gamma, IL-2 and IL-6 mRNA expressions in splenocytes and stimulate the productions of NO and TNF-alpha in peritoneal macrophages. Taken together, the three acidic polysaccharides displayed different anti-tumor activities which were associated with their different structural characteristics.
Copyright 2009 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acids
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Angelica sinensis / chemistry*
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Animals
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / pharmacology*
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Cell Proliferation / drug effects
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Chromatography, Gel
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Cytokines / genetics
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Cytokines / metabolism
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Drug Screening Assays, Antitumor
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Gene Expression Regulation / drug effects
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Longevity / drug effects
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Macrophages, Peritoneal / cytology
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / metabolism
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Male
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Mice
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Neoplasms / pathology
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Organ Size / drug effects
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Phagocytosis / drug effects
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Polysaccharides / chemistry
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Polysaccharides / pharmacology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sarcoma 180
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Spleen / cytology
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Spleen / drug effects
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Spleen / metabolism
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Spleen / pathology
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Thymus Gland / drug effects
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Thymus Gland / pathology
Substances
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Acids
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Antineoplastic Agents
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Cytokines
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Polysaccharides
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RNA, Messenger