Metabolic enzyme(s) confer imidacloprid resistance in a clone of Myzus persicae (Sulzer) (Hemiptera: Aphididae) from Greece

Pest Manag Sci. 2010 Apr;66(4):390-5. doi: 10.1002/ps.1888.

Abstract

Background: Previous studies have reported varying levels of resistance against imidacloprid in several insect species, including populations of the peach-potato aphid, Myzus persicae (Sulzer). These cases of resistance have been attributed to either target-site resistance or enhanced detoxification. In this study, a clone of M. persicae originating from Greece revealed a 60-fold resistance factor to imidacloprid.

Results: The Greek clone is compared in terms of metabolic enzyme activity and synergism profiles with other M. persicae clones showing lower imidacloprid resistance.

Conclusion: A combination of in vitro biochemical assays and in vivo differential synergism studies using PBO and a close analogue EN 16/5-1 suggests that the mechanism conferring increased resistance in this clone is primarily due to enhanced oxidase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphids / drug effects*
  • Aphids / enzymology*
  • Aphids / genetics
  • Aphids / physiology
  • Biological Assay
  • Cloning, Organism
  • Discriminant Analysis
  • Dose-Response Relationship, Drug
  • Female
  • Greece
  • Imidazoles / pharmacology*
  • Insecticide Resistance / drug effects*
  • Insecticide Resistance / genetics
  • Neonicotinoids
  • Nitro Compounds / pharmacology*
  • Piperonyl Butoxide

Substances

  • Imidazoles
  • Neonicotinoids
  • Nitro Compounds
  • imidacloprid
  • Piperonyl Butoxide