CE-MALDI interface based on inkjet technology

Electrophoresis. 2009 Dec;30(23):4071-4. doi: 10.1002/elps.200900414.

Abstract

An ink jet printer valve and a nozzle were used to deliver matrix and sample from an electrophoresis capillary onto a MALDI plate. The system was evaluated by the separation of a set of standard peptides. That separation generated up to 40 000 theoretical plates in less than 3 min. Detection limits were 500 amol for an ABI TOF-TOF instrument and 2 fmol for an ABI Q-TOF instrument. Over 70% coverage was obtained for the tryptic digest of alpha-lactalbumin in less than 2.5 min.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / chemistry
  • Electrophoresis, Capillary / instrumentation*
  • Equipment Design
  • Peptides / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation*

Substances

  • Peptides
  • Angiotensin II