Improved detection of microbial DNA after bead-beating before DNA isolation

Richard de Boer; Remco Peters; Sonja Gierveld; Tim Schuurman; Mirjam Kooistra-Smid; Paul Savelkoul

doi:10.1016/j.mimet.2009.11.009

Improved detection of microbial DNA after bead-beating before DNA isolation

J Microbiol Methods. 2010 Feb;80(2):209-11. doi: 10.1016/j.mimet.2009.11.009. Epub 2009 Dec 6.

Authors

Richard de Boer¹, Remco Peters, Sonja Gierveld, Tim Schuurman, Mirjam Kooistra-Smid, Paul Savelkoul

Affiliation

¹ Department of Research & Development, Laboratory for Infectious Diseases Groningen, The Netherlands.

PMID: 19995580
DOI: 10.1016/j.mimet.2009.11.009

Abstract

Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.

MeSH terms

Bacteriolysis
Clinical Laboratory Techniques / methods*
DNA, Bacterial / isolation & purification*
Gram-Positive Bacteria / genetics
Gram-Positive Bacteria / isolation & purification*
Gram-Positive Bacterial Infections / diagnosis*
Microspheres
Molecular Diagnostic Techniques / methods

Substances

DNA, Bacterial