Routine versus clinically driven laboratory monitoring of HIV antiretroviral therapy in Africa (DART): a randomised non-inferiority trial

Lancet. 2010 Jan 9;375(9709):123-31. doi: 10.1016/S0140-6736(09)62067-5. Epub 2009 Dec 8.

Abstract

Background: HIV antiretroviral therapy (ART) is often managed without routine laboratory monitoring in Africa; however, the effect of this approach is unknown. This trial investigated whether routine toxicity and efficacy monitoring of HIV-infected patients receiving ART had an important long-term effect on clinical outcomes in Africa.

Methods: In this open, non-inferiority trial in three centres in Uganda and one in Zimbabwe, 3321 symptomatic, ART-naive, HIV-infected adults with CD4 counts less than 200 cells per microL starting ART were randomly assigned to laboratory and clinical monitoring (LCM; n=1659) or clinically driven monitoring (CDM; n=1662) by a computer-generated list. Haematology, biochemistry, and CD4-cell counts were done every 12 weeks. In the LCM group, results were available to clinicians; in the CDM group, results (apart from CD4-cell count) could be requested if clinically indicated and grade 4 toxicities were available. Participants switched to second-line ART after new or recurrent WHO stage 4 events in both groups, or CD4 count less than 100 cells per microL (LCM only). Co-primary endpoints were new WHO stage 4 HIV events or death, and serious adverse events. Non-inferiority was defined as the upper 95% confidence limit for the hazard ratio (HR) for new WHO stage 4 events or death being no greater than 1.18. Analyses were by intention to treat. This study is registered, number ISRCTN13968779.

Findings: Two participants assigned to CDM and three to LCM were excluded from analyses. 5-year survival was 87% (95% CI 85-88) in the CDM group and 90% (88-91) in the LCM group, and 122 (7%) and 112 (7%) participants, respectively, were lost to follow-up over median 4.9 years' follow-up. 459 (28%) participants receiving CDM versus 356 (21%) LCM had a new WHO stage 4 event or died (6.94 [95% CI 6.33-7.60] vs 5.24 [4.72-5.81] per 100 person-years; absolute difference 1.70 per 100 person-years [0.87-2.54]; HR 1.31 [1.14-1.51]; p=0.0001). Differences in disease progression occurred from the third year on ART, whereas higher rates of switch to second-line treatment occurred in LCM from the second year. 283 (17%) participants receiving CDM versus 260 (16%) LCM had a new serious adverse event (HR 1.12 [0.94-1.32]; p=0.19), with anaemia the most common (76 vs 61 cases).

Interpretation: ART can be delivered safely without routine laboratory monitoring for toxic effects, but differences in disease progression suggest a role for monitoring of CD4-cell count from the second year of ART to guide the switch to second-line treatment.

Funding: UK Medical Research Council, the UK Department for International Development, the Rockefeller Foundation, GlaxoSmithKline, Gilead Sciences, Boehringer-Ingelheim, and Abbott Laboratories.

Publication types

  • Multicenter Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / analogs & derivatives
  • Adenine / therapeutic use
  • Adolescent
  • Adult
  • Africa / epidemiology
  • Aged
  • Anemia / epidemiology
  • Anti-Retroviral Agents / therapeutic use*
  • CD4 Lymphocyte Count
  • Creatinine / analysis
  • Dideoxynucleosides / therapeutic use
  • Disease Progression
  • Drug Monitoring*
  • Female
  • Glomerular Filtration Rate
  • HIV Infections / classification
  • HIV Infections / drug therapy*
  • HIV Infections / mortality
  • HIV-1 / genetics
  • HIV-Associated Lipodystrophy Syndrome / epidemiology
  • Hemoglobins / analysis
  • Humans
  • Lamivudine / therapeutic use
  • Male
  • Middle Aged
  • Neutropenia / epidemiology
  • Neutrophils / metabolism
  • Nevirapine / therapeutic use
  • Organophosphonates / therapeutic use
  • RNA, Viral / metabolism
  • Tenofovir
  • Urea / analysis
  • Viral Load
  • Zidovudine / therapeutic use

Substances

  • Anti-Retroviral Agents
  • Dideoxynucleosides
  • Hemoglobins
  • Organophosphonates
  • RNA, Viral
  • Lamivudine
  • Zidovudine
  • Urea
  • Nevirapine
  • Tenofovir
  • Creatinine
  • Adenine
  • abacavir

Associated data

  • ISRCTN/ISRCTN13968779