Imaging tumor variation in response to photodynamic therapy in pancreatic cancer xenograft models

Int J Radiat Oncol Biol Phys. 2010 Jan 1;76(1):251-9. doi: 10.1016/j.ijrobp.2009.08.041.

Abstract

Purpose: A treatment monitoring study investigated the differential effects of orthotopic pancreatic cancer models in response to interstitial photodynamic therapy (PDT), and the validity of using magnetic resonance imaging as a surrogate measure of response was assessed.

Methods and materials: Different orthotopic pancreatic cancer xenograft models (AsPC-1 and Panc-1) were used to represent the range of pathophysiology observed in human beings. Identical dose escalation studies (10, 20, and 40J/cm) using interstitial verteporfin PDT were performed, and magnetic resonance imaging with T2-weighted and T1-weighted contrast were used to monitor the total tumor volume and the vascular perfusion volume, respectively.

Results: There was a significant amount of necrosis in the slower-growing Panc-1 tumor using high light dose, although complete necrosis was not observed. Lower doses were required for the same level of tumor kill in the faster-growing AsPC-1 cell line.

Conclusions: The tumor growth rate and vascular pattern of the tumor affect the optimal PDT treatment regimen, with faster-growing tumors being relatively easier to treat. This highlights the fact that therapy in human beings shows a heterogeneous range of outcomes, and suggests a need for careful individualized treatment outcomes assessment in clinical work.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Contrast Media
  • Feasibility Studies
  • Humans
  • Magnetic Resonance Imaging / methods*
  • Male
  • Mice
  • Mice, SCID
  • Necrosis
  • Pancreatic Neoplasms / blood supply
  • Pancreatic Neoplasms / drug therapy*
  • Pancreatic Neoplasms / mortality
  • Pancreatic Neoplasms / pathology
  • Photochemotherapy / methods*
  • Photochemotherapy / mortality
  • Photosensitizing Agents / administration & dosage*
  • Porphyrins / administration & dosage*
  • Radiotherapy Dosage
  • Tumor Burden
  • Verteporfin
  • Xenograft Model Antitumor Assays

Substances

  • Contrast Media
  • Photosensitizing Agents
  • Porphyrins
  • Verteporfin