Generation of transgenic rats by microinjection of short DNA fragments

Methods Mol Biol. 2010:597:81-92. doi: 10.1007/978-1-60327-389-3_6.

Abstract

Here we describe an efficient technique to generate transgenic rats by microinjection of short DNA fragments. We have focused on optimal conditions for superovulation of prepubescent females Sprague-Dawley (CD) strains to have good quality embryos, pseudopregnant females, zygotes preparation, optimal conditions for microinjection and embryo transfer into foster mothers.

MeSH terms

  • Animals
  • DNA / administration & dosage*
  • Embryo Transfer / methods*
  • Female
  • Male
  • Microinjections / methods*
  • Ovulation Induction / methods
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Transgenic / genetics*
  • Transgenes
  • Zygote / cytology

Substances

  • DNA