Abstract
Objective:
RBL-2H3 cells express Toll-like receptors, including TLR4. This study aims to assess various aspects of the TLR4 pathway.
Methods:
RBL-2H3 cells were indirectly stained for cell surface TLR4, 25 CD14 and intracellular MyD88 proteins and analysed through flow cytometry for single-colour staining.
Results:
While TLR4-receptors are expressed in RBL-2H3 cells, associated elements involved in the signaling pathway, CD14 and MyD88, are not.
Conclusion:
Care should be taken if RBL-2H3 cells are used to study aspects of the innate immune system in mast cells.
MeSH terms
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Animals
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Cell Degranulation / drug effects
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Cell Line
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Flow Cytometry
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Fluorescent Dyes
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Lipopolysaccharide Receptors / metabolism
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Lipopolysaccharides / pharmacology
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Mast Cells / physiology*
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Myeloid Differentiation Factor 88 / metabolism
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Peptidoglycan / pharmacology
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Rats
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Salmonella enterica / chemistry
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Signal Transduction / physiology*
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Toll-Like Receptor 4 / physiology*
Substances
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Fluorescent Dyes
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Lipopolysaccharide Receptors
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Lipopolysaccharides
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Myd88 protein, rat
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Myeloid Differentiation Factor 88
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Peptidoglycan
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Tlr4 protein, rat
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Toll-Like Receptor 4