Abstract
We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Allergens*
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Amino Acid Sequence
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Antigens, Plant
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Cloning, Molecular
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DNA / genetics
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Gene Expression
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Genes, Plant
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Glycoproteins / genetics
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Molecular Sequence Data
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Plant Proteins*
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Pollen* / genetics*
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Secale / genetics*
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Secale / immunology
Substances
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Allergens
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Antigens, Plant
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Glycoproteins
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Lol p I protein, Lolium perenne
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Plant Proteins
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DNA
Associated data
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GENBANK/X57678
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GENBANK/X58797
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GENBANK/X58798
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GENBANK/X58799
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GENBANK/X58800
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GENBANK/X58801
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GENBANK/X58802
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GENBANK/X58803
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GENBANK/X62840
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GENBANK/X62841