Abstract
This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Biosensing Techniques
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Gold / chemistry*
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Luciferases / chemistry*
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Luciferases / genetics
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Luciferases / metabolism
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Luminescent Agents / chemistry*
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Matrix Metalloproteinase 2 / analysis*
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Matrix Metalloproteinase 2 / chemistry
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Matrix Metalloproteinase 2 / metabolism
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Metal Nanoparticles / chemistry*
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Mutation
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Luminescent Agents
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Recombinant Proteins
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Gold
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Luciferases
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Matrix Metalloproteinase 2