Expression and regulation of ANTXR1 in the chick embryo

Dev Dyn. 2010 Feb;239(2):680-7. doi: 10.1002/dvdy.22194.

Abstract

Anthrax Toxin Receptor 1 (ANTXR1; also known as Tumor Endothelial Marker 8, TEM8) is one of several genes that was recently found to be up-regulated in tumor-associated endothelial cells. In vitro, the protein can link extracellular matrix components with the actin cytoskeleton to promote cell adhesion and cell spreading. Both, ANTXR1 and the closely related ANTXR2 can bind anthrax toxin and interact with lipoprotein receptor-related protein 5 and 6, which also work as coreceptors in the WNT signaling pathway. Here, we report the cloning of chick ANTXR1 from a suppression subtractive hybridization screen for fibroblast growth factor (FGF) -inducible genes in chicken embryonic facial mesenchyme. We show that chicken ANTXR1 is dynamically expressed throughout embryogenesis, starting from Hamburger and Hamilton stage 10. Furthermore, we demonstrate that FGF signaling is sufficient, but not necessary, to induce ANTXR1 expression in chicken facial mesenchyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chick Embryo
  • Embryonic Development*
  • Face / embryology*
  • Fibroblast Growth Factor 8 / metabolism*
  • Gene Expression Regulation, Developmental*
  • Mesoderm / metabolism*
  • Receptors, Peptide / metabolism*
  • Signal Transduction
  • Wnt Proteins / metabolism

Substances

  • Receptors, Peptide
  • Wnt Proteins
  • anthrax toxin receptors
  • Fibroblast Growth Factor 8