Identification of spermatozoa in archived testicular cancer specimens: implications for bench side sperm retrieval at orchiectomy

Urology. 2010 Jun;75(6):1436-40. doi: 10.1016/j.urology.2009.10.039. Epub 2009 Dec 29.

Abstract

Objectives: To evaluate the patterns of spermatogenesis in the normal testicular parenchyma of primary testicular cancers and estimate the feasibility of sperm retrieval at the time of radical orchiectomy.

Methods: We reviewed the archived histologic sections of 39 consecutive patients who had undergone radical orchiectomy for primary testicular cancer at 3 university-affiliated hospitals. We examined all areas of normal (noncancerous) testicular parenchyma to evaluate the level of spermatogenesis and presence of mature spermatozoa in these sections. A minimum of 100 seminiferous tubules were scored per case. We also evaluated the epididymal tubules for the presence of mature spermatozoa. A review of the clinical chart was performed to evaluate the relationship between clinical data and histologic findings.

Results: In nearly 40% of the cases evaluated (15/38), the predominant histologic pattern was full spermatogenesis. Mature spermatozoa were identified in nearly 80% (30/38) of the testicular histologic sections and in 50% (14/28) of the evaluable epididymal sections. Clinical stage (presence of extranodal disease) and tumor marker levels were related to the probability of identifying mature spermatozoa in the testis.

Conclusions: The data suggest that sperm retrieval at the time of radical orchiectomy is a feasible fertility option, with a 40% probability of recovering spermatozoa by random biopsy of the noncancerous testicular parenchyma and an 80% probability of recovering spermatozoa with a more extensive dissection. In 50% men, spermatozoa may be recovered by epididymal aspiration alone.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Analysis of Variance
  • Cohort Studies
  • Cryopreservation
  • Epididymis / pathology
  • Feasibility Studies
  • Follow-Up Studies
  • Hospitals, University
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Orchiectomy / methods*
  • Probability
  • Registries
  • Regression Analysis
  • Risk Factors
  • Semen Preservation
  • Sperm Retrieval*
  • Spermatogenesis / physiology*
  • Spermatozoa / pathology*
  • Testicular Neoplasms / pathology
  • Testicular Neoplasms / surgery*
  • Young Adult