Abstract
Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Anaerobiosis
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Bacterial Proteins / genetics
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Citric Acid Cycle
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Escherichia coli / genetics
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Escherichia coli / growth & development*
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Escherichia coli / metabolism*
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Fermentation
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Gene Deletion
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Genes, Bacterial
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Genetic Engineering
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Glucose / metabolism
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Glyoxylates / metabolism
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Mutation
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Oxidation-Reduction
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
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Sigma Factor / genetics
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Succinic Acid / metabolism*
Substances
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Bacterial Proteins
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Glyoxylates
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Sigma Factor
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sigma factor KatF protein, Bacteria
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Succinic Acid
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphoenolpyruvate-glucose phosphotransferase
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Glucose