Design, synthesis and studies of triphosphate analogues for the production of anti AZT-TP antibodies

Camille Roucairol; Stéphane Azoulay; Marie-Claire Nevers; Jérôme Golebiowski; Christophe Créminon; Jacques Grassi; Alain Burger; Danièle Duval

doi:10.1016/j.bmcl.2009.12.053

Design, synthesis and studies of triphosphate analogues for the production of anti AZT-TP antibodies

Bioorg Med Chem Lett. 2010 Feb 1;20(3):987-90. doi: 10.1016/j.bmcl.2009.12.053. Epub 2009 Dec 21.

Authors

Camille Roucairol¹, Stéphane Azoulay, Marie-Claire Nevers, Jérôme Golebiowski, Christophe Créminon, Jacques Grassi, Alain Burger, Danièle Duval

Affiliation

¹ Laboratoire de Chimie des Molécules Bioactives et Arômes, UMR 6001 CNRS, Institut de Chimie de Nice, Université de Nice-Sophia Antipolis, Nice, France.

PMID: 20045322
DOI: 10.1016/j.bmcl.2009.12.053

Abstract

Triphosphates anabolites are the active chemical species of nucleosidic reverse transcriptase inhibitors in HIV-therapy. Herein, we describe (i) the design of stable triphosphate analogues of AZT using molecular modelling, (ii) their synthesis and (iii) their use for producing anti AZT-TP antibodies in the aim of developing an immunoassay for therapeutic drug monitoring.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibody Formation*
Dideoxynucleotides / blood
Dideoxynucleotides / immunology*
Drug Design*
Polyphosphates / blood
Polyphosphates / chemical synthesis*
Polyphosphates / immunology*
Rabbits
Thymine Nucleotides / blood
Thymine Nucleotides / immunology*
Zidovudine / analogs & derivatives*
Zidovudine / blood
Zidovudine / immunology

Substances

Dideoxynucleotides
Polyphosphates
Thymine Nucleotides
Zidovudine
zidovudine triphosphate
triphosphoric acid