Meningococcal serogroup C polysaccharide specific memory B cells, directly enumerated by labeled polysaccharide, are not affected by age at vaccination

Vaccine. 2010 Feb 25;28(9):2097-103. doi: 10.1016/j.vaccine.2009.12.039. Epub 2010 Jan 5.

Abstract

The influence of age on the generation and persistence of specific memory B cells after vaccination with Neisseria meningitidis type C polysaccharide (MenC-PS) conjugate is unknown. MenC-PS-specific B cells could be directly enumerated by fluorochrome-labeled MenC-PS and flow cytometry in blood up to at least 4 years after vaccination, ranging from 0.01% to 0.78% of total B cells and did not correlate with age at vaccination. The percentage of MenC-specific memory B cells out of total memory B cells correlated with total MenC-specific B-cells and with frequencies of IgA(+) plus IgG(+) MenC-specific AbSC, but not with MenC-specific Ab.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / immunology
  • B-Lymphocytes / immunology*
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Immunologic Memory / immunology*
  • Infant
  • Leukocytes, Mononuclear / immunology
  • Male
  • Meningococcal Infections / immunology
  • Meningococcal Infections / prevention & control*
  • Meningococcal Vaccines / immunology*
  • Middle Aged
  • Neisseria meningitidis, Serogroup C / immunology
  • Polysaccharides, Bacterial / immunology
  • Vaccines, Conjugate / immunology
  • Young Adult

Substances

  • Antibodies, Bacterial
  • Meningococcal Vaccines
  • Polysaccharides, Bacterial
  • Vaccines, Conjugate
  • meningococcal group C polysaccharide