High mass selectivity for top-down proteomics by application of SWIFT technology

J Am Soc Mass Spectrom. 2010 Mar;21(3):455-9. doi: 10.1016/j.jasms.2009.11.011. Epub 2009 Dec 11.

Abstract

Stored waveform inverse Fourier transform (SWIFT) technology has been implemented on a commercial Fourier transform ICR mass spectrometer. Complex ejection/isolation waveforms can be generated on an arbitrary waveform generator (AWG) and applied to the instrument by use of a high speed analog switch. High mass selectivity and subsequent electron capture dissociation (ECD) of the SWIFT isolated ions has been demonstrated with analysis of intact Bovine histone H4. A mass selectivity about 0.1 m/z unit for isolation of the 18+ charge state ions was achieved. Adaptation of SWIFT on the commercial instrument provides an enhanced capability for characterizing intact proteins by top-down analysis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Algorithms*
  • Equipment Design
  • Equipment Failure Analysis
  • Peptide Mapping / instrumentation*
  • Proteomics / instrumentation*
  • Proteomics / methods
  • Sensitivity and Specificity
  • Signal Processing, Computer-Assisted / instrumentation*
  • Spectrometry, Mass, Electrospray Ionization / instrumentation*
  • Spectrometry, Mass, Electrospray Ionization / methods