Abstract
Objective:
To construct adenovirus vector vaccine against H5N1 influenza virus and study on the immunogenicity.
Methods:
In this study, we amplified hemagglutinin (HA) gene sequence of H5N1 influenza virus (A/Anhui/1/2005), then constructed an adenovirus vector vaccine (Adv-HA), followed by tests in BALB/c mice for the immunogenicity with the vaccine and immunization strategies.
Results:
The recombinate Adv-HA vaccine could effectively induce both humoral and cellular immunity against human H5N1 influenza virus.
Conclusion:
The Adv-HA vaccination against H5N1 influenza is a potential strategy and worthy of further investigation.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics
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Adenoviridae / immunology
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Animals
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Cell Line
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Female
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Genetic Vectors / genetics
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Genetic Vectors / immunology
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Hemagglutinin Glycoproteins, Influenza Virus / administration & dosage
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Humans
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Influenza A Virus, H5N1 Subtype / genetics
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Influenza A Virus, H5N1 Subtype / immunology*
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / genetics
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Influenza Vaccines / immunology*
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Influenza, Human / immunology*
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Influenza, Human / prevention & control*
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Influenza, Human / virology
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Mice
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Mice, Inbred BALB C
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Random Allocation
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Vaccination
Substances
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines